Tabs on lifestyle
The brand new nutritionist facilities give a FFQ and you will an actual physical interest survey to all the participants at the beginning at the end of this new trial. One another surveys was basically normalized of the IDEFICS and HELENA Western european Ideas and you may validated by CTS-02203 Perfection Investment of your Regional Authorities from Andalucia. The fresh HELENA Investigation establish and you will examined a questionnaire for usage certainly teenagers, based on the long format of the Around the world Physical activity Survey (IPAQ) , which offered worldwide similar study .
Bloodstream, pee and fecal testing
Three milliliters of bloodstream are accumulated initially at the termination of new demo. The bloodstream try pulled via the antecubital vein. Peripheral white-blood tissue (buffy coat) try drawn for deoxyribonucleic acidic (DNA) extraction. Moreover, a 1-ml urine sample is actually received having oxidization marker studies. Students cannot consume to own a dozen times until the testing.